Biological activity of a fluorescent 12-O-tetradecanoyl-phorbol-13-acetate derivative

A newly synthesized fluorescent TPA derivative, O-(N-dansylamino-3-tetradecanoyl)-12,O-acetyl-13-phorbol (Dansyl-TPA), inhibited metabolic cooperation of Chinese hamster V79 cells in culture and also the specific binding of [3H]phorbol dibutyrate to V79 cells. Dansyl-TPA should be a valuable tool for studying the cellular targets of TPA.