Related Experiment Video
Updated: Aug 7, 2026

Thermostabilization, Expression, Purification, and Crystallization of the Human Serotonin Transporter Bound to S-citalopram
Published on: November 27, 2016
Use of [75Se]selenomethionine in immunoglobulin biosynthetic studies
Abstract:
The gamma-emitting amino acid analog, [75Se]selenomethionine, has been used as a biosynthetic label for immunoglobulins secreted by plasmacytomas in tissue culture. The secreted products are structurally intact with respect to their antibody combining sites and their class and allotype antigenic specificities. A component of [75Se]selenomethionine preparations was found to bind to fetal calf serum proteins, in a manner releasable by mercaptoethanol, but not by sodium dodecyl sulfate (SDS) and urea. Methods for circumventing the problems caused by this binding are described.
More Related Videos
Related Concept Videos
Hybridoma Technology
Hybridoma Selection
Commonly used fusion techniques — electroporation, polyethylene glycol...
Immunogold Electron Microscopy

