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Characterization of anti-double-stranded DNA antibody-forming cells in the NZB/W mouse spleen

H Okudaira, E Terada, A Ghoda

    International Archives of Allergy and Applied Immunology
    |January 1, 1983
    PubMed

    Characterization of anti-double-stranded (ds) DNA antibody-forming cells in the spleen of the NZB/W mouse was attempted. Anti-ds DNA antibody-forming cells formed antibodies in vitro without any antigenic stimulus. Depletion of T cells in the NZB/W spleen cells did not abolish but enriched the anti-ds DNA antibody-forming capacity. Anti-ds DNA antibody formation was not abolished by 1,000 R of irradiation which suggests that there was no mitotic process involved. An in vivo irradiation experiment using NZB/W mice indicated that persistent anti-ds DNA antibody formation is mediated by radio-resistant antibody-forming cells.

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