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Related Experiment Videos

Endotoxin-induced T lymphocyte proliferation.

S N Vogel, M L Hilfiker, M J Caulfield

    Journal of Immunology (Baltimore, Md. : 1950)
    |April 1, 1983
    PubMed
    Summary
    This summary is machine-generated.

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    This study reveals that T cells, not just B cells, can be directly stimulated by lipopolysaccharide (LPS). This endotoxin-sensitive T cell population plays a key role in immune responses.

    Area of Science:

    • Immunology
    • Cell Biology

    Background:

    • Lipopolysaccharide (LPS) is known to activate B lymphocytes.
    • The direct effect of LPS on T cells has not been previously established.

    Purpose of the Study:

    • To investigate the direct effect of LPS on T cells.
    • To identify and characterize LPS-responsive T cell populations.

    Main Methods:

    • Utilized a cloned murine interleukin 2-dependent cytotoxic T cell line (CT 6).
    • Purified splenic T lymphocyte populations.
    • Assessed proliferation via 3H-thymidine uptake.
    • Used polymyxin B to inhibit LPS activity.
    • Tested exogenous interleukin 1 for comparative stimulation.

    Main Results:

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    • The cloned T cell line CT 6 proliferated in response to LPS, demonstrating direct T cell stimulation.
    • A small subpopulation (3%) of purified splenic T cells also proliferated in response to LPS.
    • This LPS-induced T cell proliferation was dose-dependent, time-dependent, and inhibited by polymyxin B.
    • The responsive T cell population was absent in endotoxin-hyporesponsive C3H/HeJ mice.
    • Interleukin 1 did not account for the observed LPS-induced T cell stimulation.

    Conclusions:

    • T cells can be directly stimulated by LPS, challenging the long-held view of LPS as solely a B cell activator.
    • An endotoxin-sensitive T cell population exists and is involved in immune responses.
    • These findings have implications for understanding LPS-induced adjuvanticity and other immune functions.