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Related Experiment Videos

Complement-activating rheumatoid-factor-containing complexes in patients with rheumatoid vasculitis.

C J Elson, D G Scott, D R Blake

    Annals of the Rheumatic Diseases
    |April 1, 1983
    PubMed
    Summary
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    Rheumatoid factor (RF) immune complexes binding complement were found in high levels in rheumatoid arthritis patients with vasculitis. These findings suggest RF-containing complexes may play a role in vasculitic rheumatic diseases.

    Area of Science:

    • Immunology
    • Rheumatology
    • Clinical Chemistry

    Background:

    • Immune complexes are implicated in the pathogenesis of various rheumatic diseases.
    • Rheumatoid factor (RF) is a key component of immune complexes in rheumatoid arthritis.
    • The role of complement activation by RF-containing immune complexes in specific rheumatic disease subsets requires further elucidation.

    Purpose of the Study:

    • To investigate the presence and levels of complement-bound rheumatoid factor in immune complexes from patients with diverse rheumatic diseases.
    • To determine if elevated levels of RF-C3 complexes correlate with specific clinical manifestations, particularly vasculitis.

    Main Methods:

    • Immune complexes were assessed using F(ab)2 anti-C3 immobilized on a solid matrix to quantify bound RF.

    Related Experiment Videos

  • Serum samples from patients with rheumatoid arthritis (RA), vasculitis, synovitis, ankylosing spondylitis, and systemic lupus erythematosus (SLE) were analyzed.
  • Characterization of the immune complexes included precipitation with polyethylene glycol and ultracentrifugation.

    • Main Results:

    • High levels of RF bound to C3 were detected in patients with RA complicated by vasculitis.
    • Lower levels of RF bound to C3 were observed in patients with synovitis, ankylosing spondylitis, and SLE.
    • The detected activity was confirmed to be pre-formed immune complexes in the sera.

    Conclusions:

    • RF-containing immune complexes that bind complement are significantly elevated in patients with RA and vasculitis.
    • These findings suggest a potential in vivo role for RF-C3 complexes in the immunopathogenesis of vasculitis associated with rheumatic diseases.