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Human interleukin 1: purification and properties.

L B Lachman

    Federation Proceedings
    |June 1, 1983
    PubMed
    Summary
    This summary is machine-generated.

    Researchers purified human interleukin 1 (IL 1), a key immune signaling molecule. Purified IL 1 stimulated various immune cells and induced fever and acute phase responses in vivo, highlighting its biological significance.

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    Area of Science:

    • Immunology
    • Biochemistry

    Background:

    • Human interleukin 1 (IL 1) is a crucial cytokine involved in immune responses.
    • Previous studies have indicated the biological activities of IL 1, but homogeneous purification was challenging.

    Purpose of the Study:

    • To achieve homogeneous purification of biologically active human IL 1.
    • To characterize the biological activities of purified human IL 1 on various cell types and in vivo.

    Main Methods:

    • Purification involved molecular weight fractionation, isoelectric focusing, and preparative polyacrylamide gel electrophoresis.
    • Homogeneity was confirmed using silver staining on analytical polyacrylamide gels.
    • Biological activity was assessed by stimulating human and mouse lymphocytes, fibroblasts, and synovial cells, and by in vivo animal studies.

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    Main Results:

    • Homogeneous human IL 1 was successfully purified.
    • IL 1 purified using nondenaturing gels retained significant biological activity.
    • Stimulation of T and B lymphocytes, fibroblasts, and synovial cells was observed.
    • In vivo administration induced fever, elevated acute phase proteins, and neutrophilia.

    Conclusions:

    • Homogeneous purification of biologically active human IL 1 is achievable.
    • Purified IL 1 exhibits potent stimulatory effects on diverse immune and non-immune cells.
    • IL 1 plays a significant role in orchestrating systemic immune responses, including fever and acute phase reactions.