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Construction of an antigenic map for human B-cell precursors
Journal of Clinical Immunology
|July 1, 1983
Summary
This study characterized human pre-B cell phenotypes across age groups using monoclonal antibodies. Findings reveal a predominant L243+, BA-1+ phenotype, with variations in BA-2 and anti-B1 expression.
Area of Science:
- Immunology
- Hematology
- Cell Biology
Background:
- Pre-B cells are crucial developmental intermediates in B-cell lymphopoiesis.
- Understanding pre-B cell surface marker expression is vital for diagnosing and monitoring hematological malignancies.
Purpose of the Study:
- To delineate the immunophenotype of human pre-B cells in fetal, pediatric, and adult bone marrow.
- To assess the binding patterns of key monoclonal antibodies, including BA-1, BA-2, BA-3 (anti-CALLA), anti-B1, L243 (anti-HLA-DR), and T101.
Main Methods:
- Single-cell analysis using double fluorochrome flow cytometry.
- Evaluation of monoclonal antibody binding to pre-B cells isolated from fetal, pediatric, and adult bone marrow samples.
Main Results:
- BA-1 and anti-HLA-DR (L243) showed consistent high expression across all age groups (approx. 80% and >95%, respectively).
- BA-2 expression decreased significantly with age, from 55% in fetal to 16% in adult bone marrow.
- CD10 (CALLA) and T101 expression were low on pre-B cells, with T101 binding to a subset of fetal sIgM+ cells.
Conclusions:
- Human pre-B cells exhibit a predominant phenotype of L243+, BA-1+, anti-CALLA-, T101-.
- Phenotypic heterogeneity exists for anti-B1 and BA-2 expression, suggesting developmental or functional differences.