Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Potassium effects on bone: comparison of two model systems.

N S Krieger, P H Stern

    The American Journal of Physiology
    |September 1, 1983
    PubMed
    Summary

    Elevated potassium partially reverses ouabain inhibition of parathyroid hormone-stimulated bone resorption in mouse and rat bones. However, potassium alone stimulates resorption only in mouse calvaria, suggesting distinct mechanisms.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    Elevated incidence of fractures in women with invasive breast cancer.

    Osteoporosis international : a journal established as result of cooperation between the European Foundation for Osteoporosis and the National Osteoporosis Foundation of the USA·2015
    Same author

    Dose-dependent differential effects of risedronate on gene expression in osteoblasts.

    Biochemical pharmacology·2011
    Same author

    Antagonist minigenes identify genes regulated by parathyroid hormone through G protein-selective and G protein co-regulated mechanisms in osteoblastic cells.

    Cellular signalling·2010
    Same author

    G alpha12/G alpha13 subunits of heterotrimeric G proteins mediate parathyroid hormone activation of phospholipase D in UMR-106 osteoblastic cells.

    Endocrinology·2005
    Same author

    Cyclosporine A and FK506 induce osteoclast apoptosis in mouse bone marrow cell cultures.

    Bone·2004
    Same author

    Bone anabolic responses to mechanical load in vitro involve COX-2 and constitutive NOS.

    Connective tissue research·2004

    Area of Science:

    • Bone Biology and Physiology
    • Endocrinology
    • Cellular Signaling

    Background:

    • Parathyroid hormone (PTH) regulates bone resorption, a process influenced by various ions and signaling molecules.
    • Ouabain, a cardiac glycoside, is known to inhibit Na-K-ATPase and affect cellular processes, including bone cell activity.
    • The role of potassium (K) in modulating bone resorption and its interaction with hormonal and pharmacological stimuli requires further elucidation.

    Purpose of the Study:

    • To investigate the effects of elevated potassium on bone resorption.
    • To examine how potassium influences ouabain's inhibition of parathyroid hormone (PTH)-stimulated bone resorption.
    • To compare the responses of different bone tissues (calvaria vs. limb bones) to potassium and ouabain.

    Main Methods:

    • Organ cultures of neonatal mouse calvaria and fetal mouse/rat limb bones were utilized.
    • Bone resorption was assessed in response to elevated potassium, ouabain, and PTH.
    • Prostaglandin E2 (PGE2) production and the effects of indomethacin were measured.
    • Osmolarity effects were also evaluated in fetal rat limb bone cultures.

    Main Results:

    • Ouabain inhibited PTH-stimulated bone resorption in all tested bone types.
    • Elevated potassium partially reversed ouabain's inhibition across all systems.
    • Potassium alone stimulated resorption only in neonatal mouse calvaria, not in limb bones.
    • Potassium stimulated PGE2 production in calvaria but not limb bones; indomethacin partially blocked K-stimulated resorption.
    • Fetal rat limb bones were more sensitive to inhibition by increased osmolarity compared to mouse calvaria.

    Conclusions:

    • The reversal of ouabain inhibition by potassium is likely mediated by Na-K-ATPase activity.
    • Potassium-induced bone resorption, observed only in calvaria, suggests a distinct mechanism possibly involving prostaglandins.
    • Differential responses of bone tissues to stimuli like potassium and osmolarity highlight the complexity of bone regulation.

    Related Experiment Videos