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Retroviral Transduction of T-cell Receptors in Mouse T-cells
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The mouse T cell receptor: comparison of MHC-restricted receptors on two T cell hybridomas

J Kappler, R Kubo, K Haskins

    Cell
    |October 1, 1983
    PubMed

    The receptors for antigen plus a major histocompatibility complex (MHC) gene product on a T cell hybridoma specific for ovalbumin plus a Class II MHC product were compared with those on another T cell hybridoma, specific for a Class I MHC product. In each case receptor material was identified by a clone-specific monoclonal antibody. The two receptors proved to have very similar gross structures, being 70-85 kd proteins, and reducing to an acidic alpha-chain and a slightly basic beta-chain, each 40-43 kd. The charge of both the acidic and basic polypeptides varied between the two receptors studied, showing that variable amino acid sequences occur in both chains.

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