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The polyclonal lipopolysaccharide response is accessory-cell-dependent.

C Fernandez, E Severinson

    Scandinavian Journal of Immunology
    |October 1, 1983
    PubMed
    Summary
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    Accessory cells are crucial for optimal lipopolysaccharide (LPS) responses in immune cells. These filler cells provide essential growth stimulation for LPS-responsive cells, enhancing DNA synthesis and antibody secretion.

    Area of Science:

    • Immunology
    • Cell Biology
    • Molecular Biology

    Background:

    • Lipopolysaccharide (LPS) is a potent activator of B cells, triggering DNA synthesis and antibody secretion.
    • The optimal response to LPS is known to be influenced by various cellular components within the immune system.

    Purpose of the Study:

    • To investigate the role of accessory cells in modulating the lipopolysaccharide (LPS) response of mouse spleen cells.
    • To determine whether different cell types, including T cells and B cells, contribute to LPS responsiveness.

    Main Methods:

    • Activation of C57BL.10 mouse spleen cells with varying concentrations of LPS.
    • Measurement of DNA synthesis, IgM, and IgG secretion.
    • Co-culture experiments with thymocytes, spleen cells from LPS-non-responder strains, irradiated cells, enriched T cells, enriched B cells, and a plasmacytoma cell line.

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    Main Results:

    • The LPS response showed a sigmoid dilution curve, diminishing at low cell concentrations.
    • Addition of thymocytes or LPS-non-responder spleen cells normalized the dilution curve and increased the overall LPS response.
    • Enriched T cells restored the response, while enriched B cells did not, suggesting a T cell-dependent accessory function.
    • Filler cells provided growth-stimulating activity to LPS-responsive cells and growth-supporting activity to tumor cells.

    Conclusions:

    • The lipopolysaccharide (LPS) response is significantly dependent on accessory cells.
    • Accessory cells, particularly T cells, play a critical role in supporting B cell activation and antibody production in response to LPS.
    • Enriched B cell populations exhibit poor LPS responsiveness, which can be partially restored by accessory cells.