Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Polyamines in Trichomonas vaginalis.

E White, D Hart, B E Sanderson

    Molecular and Biochemical Parasitology
    |December 1, 1983
    PubMed
    Summary
    This summary is machine-generated.

    Trichomonas vaginalis contains high levels of putrescine, a polyamine. This parasite secretes putrescine into its culture medium, indicating a unique metabolic process.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    Population pharmacokinetics of factor IX in hemophilia B patients undergoing surgery.

    Journal of thrombosis and haemostasis : JTH·2018
    Same author

    Boundary spanning at the science-policy interface: the practitioners' perspectives.

    Sustainability science·2018
    Same author

    Correction to: A zebrafish model of foxe3 deficiency demonstrates lens and eye defects with dysregulation of key genes involved in cataract formation in humans.

    Human genetics·2018
    Same author

    A zebrafish model of foxe3 deficiency demonstrates lens and eye defects with dysregulation of key genes involved in cataract formation in humans.

    Human genetics·2018
    Same author

    Perioperative replacement therapy in haemophilia B: An appeal to "B" more precise.

    Haemophilia : the official journal of the World Federation of Hemophilia·2018
    Same author

    Changing trends in diagnosis, staging, treatment and survival in lung cancer: comparison of three consecutive cohorts in an Australian lung cancer centre.

    Internal medicine journal·2016
    Same journal

    Cloning-based analysis of MHC class II DRB variation and Enterocytozoon bieneusi association in the Amur tiger (Panthera tigris altaica).

    Molecular and biochemical parasitology·2026
    Same journal

    Ethyl acetate extract from Dinizia excelsa Ducke wood: A potential phytotherapeutic agent with antioxidant, cytotoxic and hemolytic (in normal cells), immunomodulatory, antimicrobial, antiparasitic and antitumor activities.

    Molecular and biochemical parasitology·2026
    Same journal

    Plasmodium falciparum invasion ligand gene transcript profiles in different populations.

    Molecular and biochemical parasitology·2026
    Same journal

    Exploring the Warburg effect in Chagas disease: A biochemical approach to metabolic reprogramming.

    Molecular and biochemical parasitology·2026
    Same journal

    Stage-specific differential gene expression leads to adaptive changes in the promastigote and amastigote stages of Leishmania donovani.

    Molecular and biochemical parasitology·2026
    Same journal

    De-novo discovery of posttranslational histone modifications in Schistosoma mansoni stages.

    Molecular and biochemical parasitology·2026
    See all related articles

    Area of Science:

    • Biochemistry
    • Parasitology
    • Cell Biology

    Background:

    • Polyamines like putrescine, spermidine, and spermine are crucial for cell growth and function in various organisms.
    • Trichomonas vaginalis is a flagellated protozoan parasite responsible for trichomoniasis, a common sexually transmitted infection.

    Purpose of the Study:

    • To quantify and compare the levels of putrescine, spermidine, and spermine in Trichomonas vaginalis extracts and culture media.
    • To investigate the polyamine content of T. vaginalis at different growth stages (24 and 48 hours).

    Main Methods:

    • Culturing Trichomonas vaginalis in a modified Bushby's medium.
    • Extracting polyamines from parasite cells and spent culture media.
    • Quantifying putrescine, spermidine, and spermine levels using biochemical assays.

    Related Experiment Videos

    Main Results:

    • All three polyamines were detected in T. vaginalis extracts.
    • Putrescine levels and the putrescine/spermidine ratio were significantly higher in T. vaginalis compared to other protozoa and mammalian tissues.
    • Polyamine levels per cell were higher at 24 hours than at 48 hours.
    • Spent culture media showed a substantial increase in putrescine content, suggesting secretion.

    Conclusions:

    • Trichomonas vaginalis exhibits unusually high intracellular putrescine levels.
    • The parasite actively secretes putrescine into its surrounding environment.
    • These findings highlight a unique polyamine metabolism in T. vaginalis with potential implications for parasite biology and host-pathogen interactions.