Jove
Visualize
Contact Us

Related Experiment Videos

1,25-Dihydroxyvitamin D3 increases citrate secretion from osteosarcoma cells.

P A Price, M K Williamson, S A Sloper

    The Journal of Biological Chemistry
    |February 25, 1984
    PubMed
    Summary

    Vitamin D metabolite 1,25-dihydroxyvitamin D3 significantly increases citric acid secretion in rat osteosarcoma cells. This response, along with bone Gla protein synthesis, suggests a dual role in bone mineral regulation.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    Tropical medicine teaching for combat medical technicians: experience from UK military exercise SAIF SAREEA 3.

    BMJ military health·2020
    Same author

    Corrigendum: Slowly fading super-luminous supernovae that are not pair-instability explosions.

    Nature·2016
    Same author

    Slowly fading super-luminous supernovae that are not pair-instability explosions.

    Nature·2013
    Same author

    An ultraviolet-optical flare from the tidal disruption of a helium-rich stellar core.

    Nature·2012
    Same author

    A novel explosive process is required for the gamma-ray burst GRB 060614.

    Nature·2006
    Same author

    Artery calcification in uremic rats is increased by a low protein diet and prevented by treatment with ibandronate.

    Kidney international·2006
    JoVE
    x logofacebook logolinkedin logoyoutube logo
    ABOUT JoVE
    OverviewLeadershipBlogJoVE Help Center
    AUTHORS
    Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
    LIBRARIANS
    TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
    RESEARCH
    JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
    EDUCATION
    JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
    Terms & Conditions of Use
    Privacy Policy
    Policies

    Area of Science:

    • Biochemistry
    • Endocrinology
    • Cell Biology

    Background:

    • 1,25-dihydroxyvitamin D3 is a key hormonal form of vitamin D.
    • Osteoblasts play a crucial role in bone remodeling and mineral homeostasis.
    • Citric acid and bone Gla protein are abundant organic components of bone mineral.

    Purpose of the Study:

    • To investigate the effect of 1,25-dihydroxyvitamin D3 on citric acid secretion in rat osteosarcoma cells.
    • To compare the response kinetics of citrate secretion with known vitamin D actions, such as bone Gla protein synthesis.
    • To explore the potential coordinated role of citrate and bone Gla protein in vitamin D's action on bone.

    Main Methods:

    • Treatment of rat osteosarcoma cells with varying concentrations of 1,25-dihydroxyvitamin D3.

    Related Experiment Videos

  • Measurement of citric acid secretion over time.
  • Analysis of dose-response relationships.

    • Main Results:

    • A 6- to 10-fold increase in citric acid secretion was observed in response to 1,25-dihydroxyvitamin D3.
    • The time course for half-maximal response was 12 hours, consistent with steroidal hormone action.
    • Physiological concentrations of 1,25-dihydroxyvitamin D3 elicited the citrate response, with half-maximal effect at 0.03 ng/ml.
    • The kinetics and dose-dependence of citrate secretion paralleled those of bone Gla protein synthesis.

    Conclusions:

    • 1,25-dihydroxyvitamin D3 stimulates citric acid secretion in osteoblastic cells.
    • The parallel induction of citrate and bone Gla protein suggests a coordinated function in bone.
    • These findings indicate a potential mechanism for vitamin D's influence on the bone mineral phase.