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Effect of triiodothyronine (T3) on ribonucleic acid polymerase activity in developing tadpole hindlimb
The International Journal of Biochemistry
|January 1, 1984
Abstract:
Optimum conditions were studied for the determination of RNA polymerase activity in nuclei isolated from Rana catesbeiana tadpole hindlimbs. Tadpole nuclei were tested at 15 degrees C in the presence of spermidine (1.5 mM) bovine serum albumin (1.0%) and a high concentration of nucleoside triphosphates (1.0 mM). Tadpole nuclei exhibited a 60-70% higher total RNA polymerase activity with maximum activity of RNA polymerase I at 4 hr and RNA polymerase II at 8 hr after triiodothyronine injection. The results support a nuclear mechanism for the differentiation of tadpole hindlimbs induced by triiodothyronine.