Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Androgen binding in human fetal amnion.

F De Cicco Nardone, G M D'Aurizio, P Mori

    Journal of Steroid Biochemistry
    |January 1, 1984
    PubMed
    Summary
    This summary is machine-generated.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    FMD empty capsids combined with the Immunostant Particle Adjuvant -ISPA or ISA206 induce protective immunity against foot and mouth disease virus.

    Virus research·2021
    Same author

    Design and fabrication of novel polymeric biodegradable stents for small caliber blood vessels by computer-aided wet-spinning.

    Biomedical materials (Bristol, England)·2017
    Same author

    Role of transvaginal ultrasound in evaluation of ureteral involvement in deep infiltrating endometriosis.

    Ultrasound in obstetrics & gynecology : the official journal of the International Society of Ultrasound in Obstetrics and Gynecology·2017
    Same author

    Interactions between heme and tau-derived R1 peptides: binding and oxidative reactivity.

    Dalton transactions (Cambridge, England : 2003)·2016
    Same author

    Influence of FBG sensors length on temperature measures in laser-irradiated pancreas: theoretical and experimental evaluation.

    Annual International Conference of the IEEE Engineering in Medicine and Biology Society. IEEE Engineering in Medicine and Biology Society. Annual International Conference·2013
    Same author

    Intracerebral haematoma following spinal anaesthesia for caesarean section: case report.

    Il Giornale di chirurgia·2009

    Human fetal amnion cytosol contains specific binding sites for androgens like testosterone. This suggests a potential role for androgens in fetal membrane function during pregnancy.

    Area of Science:

    • Endocrinology
    • Reproductive Biology
    • Biochemistry

    Background:

    • The human fetal amnion plays a crucial role in pregnancy maintenance and fetal development.
    • Androgens are known to influence various physiological processes, but their specific roles in the fetal amnion are not fully understood.

    Purpose of the Study:

    • To investigate the presence and characteristics of androgen binding proteins in human fetal amnion cytosol.
    • To determine the affinity and capacity of these binding sites for testosterone and its metabolites.

    Main Methods:

    • Cytosol from human fetal amnion at term was prepared and treated to remove interfering substances.
    • Radioligand binding assays were performed using [3H]testosterone, [3H]dihydrotestosterone, and [3H]methyltrienolone.
    • Scatchard plot analysis was used to characterize the binding kinetics (affinity and capacity).

    Related Experiment Videos

    Main Results:

    • Human fetal amnion cytosol specifically binds [3H]testosterone with high affinity and low capacity.
    • Binding characteristics varied with incubation conditions, indicating complex interactions.
    • Specific binding sites for [3H]dihydrotestosterone and [3H]methyltrienolone were also identified.

    Conclusions:

    • Human fetal amnion cytosol at term possesses specific androgen-binding proteins.
    • These findings suggest a potential role for androgens in the physiology of the fetal amnion.