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Sodium transport in Rana pipiens gastric mucosa
Summary
Sodium transport across frog gastric mucosa was not observed at neutral pH but occurred with amphotericin B. This finding impacts electrolyte physiology and existing models of gastric transport.
Area of Science:
- Physiology
- Gastroenterology
- Membrane Transport
Background:
- Previous studies on mammalian gastric mucosa showed net sodium transport.
- Frog gastric mucosa studies often used low pH solutions, potentially confounding results.
- The Hogben three-variable model is a key framework for understanding gastric electrolyte physiology.
Purpose of the Study:
- To re-examine frog gastric mucosa transport properties in conditions similar to mammalian studies.
- To investigate the role of sodium transport in frog gastric electrolyte physiology.
- To assess the influence of pH and amphotericin B on frog gastric mucosa transport.
Main Methods:
- In vitro studies of frog gastric mucosa.
- Utilizing well-buffered, near-neutral pH solutions for mucosal bathing.
- Addition of amphotericin B (5 X 10(-4) M) to the mucosal solution.
- Measurement of net sodium transport and short-circuit current.
Main Results:
- No net sodium transport was observed across frog gastric mucosa at near-neutral pH.
- Addition of amphotericin B induced net sodium transport.
- Amphotericin B also caused an increase in short-circuit current.
Conclusions:
- Sodium transport can occur in frog gastric mucosa under specific experimental conditions.
- The findings suggest sodium transport plays a role in frog gastric electrolyte physiology.
- These results may necessitate a re-evaluation of the applicability of the Hogben three-variable model to frog gastric mucosa.