Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Specific antibody synthesis in vitro. I. Technical considerations.

A L Moore, W B Ershler, M P Hacker

    Journal of Immunological Methods
    |May 11, 1984
    PubMed
    Summary

    This study introduces a simplified one-step assay for measuring specific antibody production in vitro. This new method, combining microculture and enzyme-linked immunoassay, bypasses the need for mitogens and multiple steps, aiding immune competence assessment.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    Mn-porphyrins in a four-helix bundle participate in photo-induced electron transfer with a bacterial reaction center.

    Photosynthesis research·2023
    Same author

    Occurrence and greater intensity of estrus in recipient lactating dairy cows improve pregnancy per embryo transfer.

    Journal of dairy science·2021
    Same author

    The incorporation of amino acids into the protein of isolated soya bean mitochondria.

    Planta·2014
    Same author

    An estimation of the proton conductance of the inner membrane of turnip (Brassica napus L.) mitochondria.

    Planta·2014
    Same author

    Metabolite fluxes across the inner membrane of plant mitochondria - inhibition by phthalonic acid.

    Planta·2013
    Same author

    Ultrafast energy transfer and excited state coupling in an artificial photosynthetic antenna.

    The journal of physical chemistry. B·2013

    Area of Science:

    • Immunology
    • Cellular Biology
    • Biotechnology

    Background:

    • Traditional in vitro antibody production assays are complex, often requiring mitogens and multiple steps.
    • Assessing specific antibody synthesis is crucial for understanding immune responses and competence.

    Purpose of the Study:

    • To present a simplified, one-step assay for measuring specific antibody production in vitro.
    • To combine microculture and enzyme-linked immunoassay (ELISA) techniques for enhanced efficiency.

    Main Methods:

    • Peripheral blood mononuclear cells from immunized subjects were cultured in antigen-coated wells.
    • Specific antibody produced in vitro was measured using an enzyme-linked immunoassay.
    • T cell roles were investigated through separation and remixture experiments.

    Related Experiment Videos

    Main Results:

    • The novel assay successfully measured specific antibody synthesis in vitro without mitogens.
    • Antibody production was not observed in frozen-thawed lymphocytes or in the presence of puromycin.
    • T cells were found to significantly augment specific antibody production in this assay.

    Conclusions:

    • The developed assay is a simplified, efficient method for assessing in vitro specific antibody production.
    • The assay confirms that antibody synthesis is an in vitro process involving cell-cell interactions, particularly T cells.
    • This method holds potential for evaluating immune competence and the effects of biologic response modifiers.