[MIC test of Campylobacter jejuni/coli]

Area of Science:

• Microbiology
• Antimicrobial Susceptibility Testing

Background:

• Campylobacter species (C. jejuni and C. coli) are significant causes of bacterial gastroenteritis.
• Understanding their antimicrobial susceptibility is crucial for effective treatment and combating resistance.

Purpose of the Study:

• To quantitatively assess the susceptibility of clinical isolates of Campylobacter jejuni and Campylobacter coli to 12 antibacterial agents.
• To evaluate the impact of different culture media and cell concentrations on Minimum Inhibitory Concentration (MIC) determination.

Main Methods:

• Susceptibility testing of 82 C. jejuni and 6 C. coli isolates against 12 antibiotics.
• Comparison of Mueller-Hinton (M-H) agar, M-H agar with defibrinated horse blood, and M-H agar with lysed horse blood.
• MIC determination using McFarland unit 0.5 and 100-times dilution cell concentrations.

Main Results:

• No significant difference in susceptibility was observed between the three tested media.
• Minor variations in MICs were noted between McFarland unit 0.5 and 100-times dilution for some strains, notably erythromycin.
• Highest susceptibility was observed for gentamicin and amikacin; lowest for benzylpenicillin, carbenicillin, and cephaloridine.
• Minocycline exhibited a bimodal susceptibility distribution.

Conclusions:

• Standard M-H agar is suitable for Campylobacter susceptibility testing.
• Gentamicin and amikacin are effective against the tested Campylobacter isolates.
• Emerging resistance patterns, particularly for minocycline, warrant ongoing surveillance.