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Differentiation of ovine immature B cells upon exposure to phorbol myristate acetate
Immature B cells obtained from the ileal Peyer's patches ( IPPs ) of sheep, upon exposure to phorbol myristate acetate (PMA), expressed 20-30 times more sIgM per cell than nonstimulated IPP cells, and the sIgM level was the same as that of peripheral B cells. The exposure of IPP cells to PMA also induced IgM secretion. Macrophages were not required for the terminal differentiation of IPP cells in vitro.
Immature B cells obtained from the ileal Peyer's patches ( IPPs ) of sheep, upon exposure to phorbol myristate acetate (PMA), expressed 20-30 times more sIgM per cell than nonstimulated IPP cells, and the sIgM level was the same as that of peripheral B cells. The exposure of IPP cells to PMA also induced IgM secretion. Macrophages were not required for the terminal differentiation of IPP cells in vitro.