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Published on: November 4, 2014
Optimal conditions for plaque assay of echoviruses in human embryonic lung fibroblast cells
Abstract:
The present paper gives optimal conditions for plaque assays tn human embryonic lung fibroblast cells with 45 echoviruses including 11 fresh isolates, covering 31 serotypes. All the viruses tested except echovirus type 23 produced plaques at a high titer in the same cells by the methods described in this communication. Plaque formations by a number of strains were markedly enhanced in size or number or both by the addition of polycation.
Insights
This study details optimal conditions for echovirus plaque assays in human embryonic lung fibroblast cells. Polycations significantly enhanced plaque formation for most tested echovirus serotypes.
Area of Science:
- Virology
- Cell Biology
Background:
- Plaque assays are crucial for quantifying viral infectivity.
- Optimizing conditions for echovirus plaque assays is essential for accurate viral characterization.
Purpose of the Study:
- To establish optimal conditions for plaque assays using human embryonic lung fibroblast cells.
- To evaluate plaque formation across 31 echovirus serotypes, including fresh isolates.
- To investigate the effect of polycations on echovirus plaque formation.
Main Methods:
- Human embryonic lung fibroblast cells were used for plaque assays.
- 45 echovirus strains, representing 31 serotypes, were tested.
- The impact of polycation addition on plaque size and number was assessed.
Main Results:
- Optimal plaque assay conditions were determined for human embryonic lung fibroblast cells.
- Most echoviruses (excluding type 23) produced plaques at high titers.
- Polycations significantly enhanced the size and/or number of plaques for several echovirus strains.
Conclusions:
- The described methods provide optimal conditions for echovirus plaque assays in human embryonic lung fibroblast cells.
- Polycations represent a valuable tool for enhancing echovirus plaque assays, improving sensitivity and visualization.
- These findings contribute to the accurate characterization and study of echoviruses.
