Esterase patterns of Anopheles dirus (Bangkok strain) and Anopheles balabacensis (Perlis form) in the laboratory

Esterase isoenzyme were performed in Anopheles balabacensis (Perlis Form) and Anopheles dirus (Bangkok Strain and Kanchanaburi Strain). The zymogram showed four positions, E1, E2, E3 and E4. Seventy-five percent of An. balabacensis (Perlis Form) or An. dirus (Bangkok Strain) can be correctly diagnosed by the presence of fast band in E3 or super slow band in E3 respectively. Homozygous slow band of E3 cannot be fully distinguished in both species, but by the combination of E2 and E3, 99.42% can be correctly identified to differentiate species.