Potentiation of the canine lymphocyte blastogenic response by indomethacin

Cultivation of normal canine peripheral blood lymphocytes in the presence of indomethacin, a potent prostaglandin synthetase inhibitor, produced a consistent potentiation of their in vitro response to mitogenic stimulation as compared to cultures without indomethacin. The enhancing effect of indomethacin increased with decreasing concentrations of mitogens. Indomethacin had to be added to the cultures during the first four hours for the potentiating effect to be observed. These data suggest that prostaglandins may play an important role in the regulation of the dog's immune response.