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A quantitative method for determination of 5,6-dihydroxyindole-2-carboxylic acid using high-pressure liquid
Analytical Biochemistry
|June 1, 1983
Abstract:
A method for the determination of the urinary melanocyte metabolite 5,6-dihydroxyindole-2-carboxylic acid is described. The catecholic indole is adsorbed onto alumina, chromatographed on a reverse-phase octadecyl silica column, and detected by fluorometry. The sensitivity of the method permits detection of 1 pmol injected.