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Updated: Aug 1, 2026
![Automated Radiochemical Synthesis of [18F]3F4AP: A Novel PET Tracer for Imaging Demyelinating Diseases](/_next/image?url=https%3A%2F%2Fcloudfront.jove.com%2FCDNSource%2Fteasers%2F55537.jpg&w=3840&q=50)
Automated Radiochemical Synthesis of [18F]3F4AP: A Novel PET Tracer for Imaging Demyelinating Diseases
Published on: May 29, 2017
Sensitive fluorographic detection of 3H and 14C on chromatograms using methyl anthranilate as a scintillant
Abstract:
Methyl anthranilate is a simple, sensitive, and inexpensive liquid scintillant for fluorographic detection of weak beta-emitting isotopes on chromatograms. Detection of tritium is enhanced 1000-fold compared to autoradiography in a 24-h exposure. Since methyl anthranilate is a viscous liquid, it is easily applied as an even coating which subsequently solidifies at the low temperature (-80 degrees C) used for fluorography. Of several liquid scintillants tested, methyl anthranilate was most effective, followed by 9-ethyl fluorene, methyl salicylate, and 1-methyl naphthalene. The efficiency of 1-methyl naphthalene could be raised to the level of methyl anthranilate by the addition of a small amount (0.5%) of 2,5-diphenyloxazole (PPO).
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