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Ocular tissue absorption of minocycline in the rabbit
Abstract:
After intramuscular (IM) administration of minocycline hydrochloride, concentrations of the drug that would be adequate for the control of sensitive organisms (greater than 0.5 micrograms/g of tissue) were detectable in the cornea, iris, and retinochoroid 3, 6, and 12 hours after a single injection. The concentration of the drug in the aqueous humor was greater than in the vitreous humor. Following a single IM injection of 40 mg/kg, the drug level was found to be significantly higher in the iris and aqueous humor of pigmented rabbits than in the corresponding tissues of albino rabbits. The drug seems to concentrate in the pigmented layers of the ocular tissue.
Insights
Minocycline hydrochloride administered intramuscularly reaches therapeutic levels in ocular tissues like the cornea and iris for up to 12 hours. Pigmented rabbits showed higher minocycline concentrations in ocular tissues compared to albino rabbits.
Area of Science:
- Ophthalmology
- Pharmacology
- Microbiology
Background:
- Minocycline hydrochloride is an antibiotic used to treat bacterial infections.
- Understanding its distribution in ocular tissues is crucial for treating eye infections.
Purpose of the Study:
- To determine the concentration and distribution of minocycline hydrochloride in ocular tissues after intramuscular administration.
- To compare drug levels in pigmented versus albino rabbits.
Main Methods:
- Intramuscular injection of minocycline hydrochloride (40 mg/kg) in rabbits.
- Quantification of drug concentrations in cornea, iris, retinochoroid, aqueous humor, and vitreous humor at 3, 6, and 12 hours post-injection.
- Comparison of tissue concentrations between pigmented and albino rabbits.
Main Results:
- Adequate concentrations (>0.5 µg/g) for controlling sensitive organisms were found in the cornea, iris, and retinochoroid for up to 12 hours.
- Aqueous humor concentrations exceeded vitreous humor concentrations.
- Pigmented rabbits exhibited significantly higher drug levels in the iris and aqueous humor compared to albino rabbits.
- Minocycline appears to concentrate in pigmented ocular tissues.
Conclusions:
- Intramuscular minocycline hydrochloride achieves therapeutic concentrations in key ocular tissues, suggesting potential efficacy for ocular infections.
- The concentration of minocycline in ocular tissues is influenced by pigmentation, with higher levels in pigmented rabbits.