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The nucleotide sequence of methionine elongator tRNA from wheat germ
Biochemical and Biophysical Research Communications
|August 12, 1983
Abstract:
Wheat germ methionine elongator tRNA (tRNAmMet) was purified by three column chromatogaphies followed by electrophoresis on polyacrylamide gel. Its sequence is pG-G-G-G-U-G-G-U-m1G-m2G-C-G-C-A-G-D-D-G-G-C-acp3U-A-G-C-G-C-m22G-psi-A-G-G- psi-C-U-Cm-A-U-mt6A-A-psi-C-C-U-G-A-G-m7G-D-m5C-G-A-G-A-T-psi-C-G-m1A-G2-C-C-U- C-U-C-U-C-A-C-C-C-C-A-C-C-A. Two hypermodified nucleosides, methylthreoninocarbonyladenosine (mt6A) and 3(3-amino-3-carboxypropyl)uridine (acp3U), are present in this tRNA.