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Related Experiment Videos

Sickling and Vinblastine.

G Ricci, G Zavagli

    Biomedicine & Pharmacotherapy = Biomedecine & Pharmacotherapie
    |January 1, 1983
    PubMed
    Summary
    This summary is machine-generated.

    Vinblastine (VBL) at low doses reveals susceptible sickle anaemia (SS) erythrocytes prone to irreversible sickling (IS). This occurs due to overlapping membrane cation transport disorders in both VBL-treated and irreversibly sickled cells.

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    Area of Science:

    • Hematology
    • Cell Biology
    • Pharmacology

    Background:

    • Sickle cell disease (SCD) is characterized by abnormal hemoglobin leading to red blood cell (RBC) deformation.
    • Irreversible sickling (IS) is a key pathological feature of SCD, contributing to vaso-occlusion.
    • Understanding factors influencing RBC membrane properties in SCD is crucial for therapeutic development.

    Purpose of the Study:

    • To investigate the effect of low-dose Vinblastine (VBL) on sickle anaemia (SS) erythrocytes.
    • To determine if VBL can identify SS erythrocytes susceptible to irreversible sickling (IS).
    • To explore the underlying mechanisms of VBL-induced changes in SS erythrocytes.

    Main Methods:

    • Incubation of SS erythrocytes with low concentrations of Vinblastine (VBL) (0.2 mM).

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  • Comparison of erythrocyte swelling induced by VBL with IS after deoxygenation.
  • Analysis of membrane cationic traffic in VBL-treated and irreversibly sickled erythrocytes.

    • Main Results:

    • Low-dose VBL (0.2 mM) induced swelling in a percentage of SS erythrocytes overlapping with those showing IS.
    • VBL treatment revealed SS erythrocytes most liable to IS, suggesting shared membrane cation transport defects.
    • Increased VBL concentrations led to an exponential increase in SS erythrocyte swelling.
    • VBL-incubated SS erythrocytes failed to sickle upon deoxygenation, indicating altered membrane properties.

    Conclusions:

    • Vinblastine at sub-spherostomatocytosis inducing doses can identify SS erythrocytes prone to IS.
    • Overlapping disorders in membrane cationic traffic are implicated in both VBL-induced swelling and IS.
    • VBL interacts with RBC membrane subunits, altering surface properties and preventing further morphological changes like sickling.