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Related Experiment Videos

Colorimetric determination of acetaminophen.

R N Gupta, R Pickersgill, M Stefanec

    Clinical Biochemistry
    |August 1, 1983
    PubMed
    Summary

    This study presents a rapid colorimetric method for emergency acetaminophen determination in plasma. The procedure efficiently removes interfering substances, enabling quick and accurate drug level assessment.

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    Area of Science:

    • Clinical Chemistry
    • Analytical Chemistry
    • Toxicology

    Background:

    • Accurate and rapid acetaminophen quantification in plasma is crucial for clinical management, especially in emergency overdose cases.
    • Existing methods may be time-consuming or lack specificity, necessitating a more efficient emergency procedure.

    Purpose of the Study:

    • To develop and describe a novel colorimetric procedure for the emergency determination of acetaminophen in plasma.
    • To establish a method that effectively removes common interfering substances found in plasma samples.

    Main Methods:

    • Acetaminophen was extracted from plasma into ethyl acetate at physiological pH.
    • The extract underwent purification using Fuller's earth to remove bilirubin and anhydrous sodium sulfate to eliminate water.
    • The purified extract was back-extracted into carbonate and reacted with Folin-Ciocalteau reagent to form a stable colorimetric complex.

    Main Results:

    • The described method allows for the emergency determination of acetaminophen in plasma.
    • The extraction and purification steps effectively removed interfering compounds such as salicylate, amino acids, polar compounds, bilirubin, proteins, and uric acid.
    • A stable color complex was produced, suitable for quantitative analysis.

    Conclusions:

    • The developed colorimetric procedure is suitable for the rapid and emergency determination of acetaminophen in plasma.
    • This method offers a practical approach for clinical laboratories dealing with potential acetaminophen toxicity.
    • The procedure's efficiency in removing interfering substances ensures reliable results.

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