Chicken heart citrate synthase: some mechanism studies

Some mechanism studies on chicken and pig citrate synthase are described. Gibacron Blue F3GA apparently binds into both the oxaloacetate and the acetyl-CoA subsites of the enzyme. Protection by ligands against urea-induced denaturation indicates that several di(tri)-carboxylic acids bind into the oxaloacetate subsite, whereas ATP, but not Mg2+ ATP, binds into the acetyl-CoA subsite. Oxaloacetate, citrate and D-malate induce a transconformation in the enzyme, whereas alpha-ketoglutarate, L-malate and succinate do not.