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Isotope effects of tritium in a ligand-antibody binding
The International Journal of Applied Radiation and Isotopes
|July 1, 1983
Abstract:
The affinity of testosterone labelled with 1, 2, 4 or 6 tritium atoms to testosterone antisera was examined. Scatchard plots indicated an increase of equilibrium constants with the number of tritium atoms per testosterone molecule. This behaviour was observed with all 3 antisera tested. It is speculated that the increase of affinity is due to a decrease of the amplitude of vibrations of tritium atoms, resulting in a decrease of vibrational repulsions.