Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Hemin-mediated DNA strand scission.

R L Aft, G C Mueller

    The Journal of Biological Chemistry
    |October 10, 1983
    PubMed
    Summary
    This summary is machine-generated.

    Hemin (ferric protoporphyrin IX chloride) induces DNA strand breaks in the presence of oxygen and a reducing agent. Divalent cations influence this DNA degradation, with implications for gene expression during cell differentiation.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    Effects of neoadjuvant chemotherapy with or without zoledronic acid on pathological response: A meta-analysis of randomised trials.

    European journal of cancer (Oxford, England : 1990)·2016
    Same author

    Right heart failure in pregnant women with cyanotic congenital heart disease--The good, the bad and the ugly.

    International journal of cardiology·2015
    Same author

    Pediatric patients with Marfan syndrome: frequency of dural ectasia and its correlation with common cardiovascular manifestations.

    RoFo : Fortschritte auf dem Gebiete der Rontgenstrahlen und der Nuklearmedizin·2013
    Same author

    Effect of (Neo)adjuvant zoledronic acid on disease-free and overall survival in clinical stage II/III breast cancer.

    British journal of cancer·2012
    Same author

    Quality of life over time in women diagnosed with ductal carcinoma in situ, early-stage invasive breast cancer, and age-matched controls.

    Breast cancer research and treatment·2012
    Same author

    Differentiation of the biological activities of phytohaemagglutinin affecting leucocytes.

    Nature·2010

    Area of Science:

    • Biochemistry
    • Molecular Biology
    • Genetics

    Background:

    • Hemin (ferric protoporphyrin IX chloride) is involved in cellular processes.
    • DNA integrity is crucial for cellular function and gene expression.

    Purpose of the Study:

    • To investigate the DNA-damaging potential of hemin.
    • To elucidate the reaction mechanism and factors influencing hemin-induced DNA damage.

    Main Methods:

    • Incubation of supercoiled plasmid DNA with hemin, oxygen, and 2-mercaptoethanol.
    • Analysis of DNA structural changes (strand scission, degradation) using gel electrophoresis.
    • Assessment of the effect of various divalent cations (Mg2+, Ca2+, Co2+, Zn2+, Ni2+, Cu2+) on the reaction.

    Main Results:

    Related Experiment Videos

    • Hemin causes DNA strand scission, converting supercoiled DNA to open circular and linear forms.
    • Prolonged incubation leads to significant DNA degradation.
    • Magnesium (Mg2+) and calcium (Ca2+) ions inhibit hemin-induced DNA damage.
    • Transition metals (Co2+, Zn2+, Ni2+, Cu2+) promote DNA degradation by hemin.

    Conclusions:

    • Hemin actively damages DNA under specific conditions.
    • Divalent cations play a critical role in modulating hemin's effect on DNA.
    • These findings offer insights into hemin's role in regulating gene expression during cell differentiation.