Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Protein loss during nuclear isolation.

P L Paine, C F Austerberry, L J Desjarlais

    The Journal of Cell Biology
    |October 1, 1983
    PubMed
    Summary
    This summary is machine-generated.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    Hyperfractionated Cyclophosphamide and Dexamethasone Alone or in Combination with Daratumumab and/or Carfilzomib for the Treatment of Relapsed or Refractory Multiple Myeloma: A Single-Center Retrospective Analysis.

    Clinical lymphoma, myeloma & leukemia·2023
    Same author

    Thalidomide-induced toxic epidermal necrolysis.

    Pharmacotherapy·1999
    Same author

    A domain distinct from nucleoplasmin's nuclear localization sequence influences its transport.

    Biochemical and biophysical research communications·1997
    Same author

    A recommended occupational exposure limit for formaldehyde based on irritation.

    Journal of toxicology and environmental health·1997
    Same author

    Distinct phosphorylation sites differentially influence facilitated transport of an NLS-protein and its subsequent intranuclear binding.

    Biochemical and biophysical research communications·1995
    Same author

    Intranuclear binding of nucleoplasmin.

    Journal of cellular biochemistry·1995
    Same journal

    A pan-vertebrate signaling motif controls the molecular function of intracellular AQP12.

    The Journal of cell biology·2026
    Same journal

    Synergistic assembly, disassembly, and protection of complex forms of bundled F-actin.

    The Journal of cell biology·2026
    Same journal

    Recruitment and release of XPG during NER is controlled by pre- and post-incision factors and EXO1.

    The Journal of cell biology·2026
    Same journal

    Meiotic CENP-C supports centromere assembly and kinetochore recruitment in spermatogenesis.

    The Journal of cell biology·2026
    Same journal

    Phosphatidylserine and RhoB connect PI4P and PA metabolism to maintain plasma membrane identity.

    The Journal of cell biology·2026
    Same journal

    PIKfyve influences inter-organelle contacts with lysosomes to modulate the endoplasmic reticulum.

    The Journal of cell biology·2026
    See all related articles

    Cryomicrodissection allows accurate measurement of amphibian oocyte nuclear protein content. Isolated nuclei rapidly lose 95% of their protein in aqueous solutions, indicating significant protein loss in other isolation methods.

    Area of Science:

    • Cell Biology
    • Molecular Biology
    • Developmental Biology

    Background:

    • Accurate quantification of nuclear protein content is crucial for understanding cellular processes.
    • Previous methods for isolating nuclei often lead to protein loss, complicating analysis.
    • A reliable baseline for nuclear protein content has been lacking.

    Purpose of the Study:

    • To establish a method for measuring the total in vivo protein content of amphibian oocyte nuclei.
    • To provide a baseline for assessing protein loss during nuclear isolation.
    • To quantify the rate of protein loss from isolated nuclei in aqueous media.

    Main Methods:

    • Cryomicrodissection was employed to isolate intact amphibian oocyte nuclei.
    • The protein content of isolated nuclei was measured.

    Related Experiment Videos

  • The kinetics of protein loss from isolated nuclei in aqueous medium were determined.

    • Main Results:

    • Cryomicrodissection enables the measurement of the complete in vivo protein content of amphibian oocyte nuclei.
    • Isolated oocyte nuclei lost 95% of their protein when placed in an aqueous medium.
    • The half-time for this protein loss was measured to be 250 seconds.

    Conclusions:

    • Cryomicrodissection provides a vital baseline for estimating protein loss in other nuclear isolation techniques.
    • Aqueous isolation of nuclei results in substantial and rapid protein loss.
    • This finding has implications for the study of protein dynamics in isolated cell nuclei.