Determination of catecholamines in human serum by micro high-performance liquid chromatography with micro precolumn...

A dual electrochemical detector having two working electrodes (anode and cathode) in parallel--opposed configuration suitable for micro high-performance liquid chromatography was developed for the selective and sensitive detection of catecholamines on the basis of their electrochemical reversibility and catalytic amplification by recycling oxidation and re-reduction. The micro high-performance liquid chromatographic system with a micro alumina precolumn for enriching catecholamines and the dual electrochemical detector in parallel--opposed configuration was successfully utilized for the determination of catecholamines in healthy human serum injected directly after ultrafiltration.