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Related Experiment Videos

Clostridium difficile toxin A in infants.

J M Libby, S T Donta, T D Wilkins

    The Journal of Infectious Diseases
    |September 1, 1983
    PubMed
    Summary

    Clostridium difficile toxin A was detected in the feces of hospitalized infants, even those without gastrointestinal symptoms. Toxin A presence in infants correlated with toxin B, suggesting a potential role in disease, even asymptomatically.

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    Area of Science:

    • Microbiology
    • Toxicology
    • Pediatrics

    Background:

    • Clostridium difficile produces two toxins: toxin A and toxin B.
    • The role of these toxins in human pseudomembranous colitis is not fully understood.
    • C difficile is typically found in low numbers in healthy adults but can be present in infants without adverse effects.

    Purpose of the Study:

    • To investigate the presence of C difficile toxin A in hospitalized infants without obvious gastrointestinal distress.
    • To determine if toxin A is present in infants and its relationship with toxin B.

    Main Methods:

    • An Enzyme-Linked Immunosorbent Assay (ELISA) was used to detect toxin A in fecal specimens.
    • 21 hospitalized infants without gastrointestinal symptoms were tested.
    • Toxin levels were correlated with clinical presentation.

    Main Results:

    • Toxin A was detected in the feces of 10 out of 21 infants tested.
    • No infant had toxin A without also having toxin B.
    • High titers of toxin B generally corresponded with high concentrations of toxin A.
    • One infant with antibiotic-associated colitis had the highest titers of both toxins.

    Conclusions:

    • C difficile toxin A can be present in the feces of hospitalized infants, including those without enteric symptoms.
    • The presence of toxin A in infants is always associated with toxin B.
    • Further research is needed to understand the clinical significance of toxin A and B in asymptomatic infants.

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