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Related Experiment Videos

Metal-binding ligands in Viokase.

M T Martin, F A Jacobs, J G Brushmiller

    Journal of Pediatric Gastroenterology and Nutrition
    |January 1, 1983
    PubMed
    Summary

    This study investigated zinc binders in Viokase, a treatment for acrodermatitis enteropathica (AE). Researchers found amino acids, not citrate or picolinate, likely bind zinc, suggesting these compounds are not the primary zinc transporters in AE treatment.

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    Area of Science:

    • Biochemistry
    • Nutritional Science
    • Pharmacology

    Background:

    • Viokase is a porcine pancreatic preparation used to treat acrodermatitis enteropathica (AE).
    • The precise mechanism of zinc binding and transport in Viokase is not fully understood.
    • Low molecular weight zinc binders are suspected to play a role in AE treatment.

    Purpose of the Study:

    • To identify and characterize low molecular weight zinc-binding ligands in Viokase.
    • To evaluate the potential role of citrate and picolinate as zinc transporters in Viokase.
    • To elucidate the mechanism of zinc delivery in the treatment of variant AE.

    Main Methods:

    • Ultrafiltration of Viokase extracts to isolate solutes ≤ 500 daltons.
    • Modified gel chromatography (MGC) using Sephadex G-15-120 columns with copper or zinc buffers.
    • Atomic absorption spectroscopy for metal analysis and various techniques for amino acid quantification.

    Main Results:

    • MGC revealed peaks corresponding to glutamic acid, other amino acids, and metal ions.
    • Citrate and picolinate were not detected as significant zinc binders by MGC.
    • Small amounts of citrate-like compounds were detected, but their role in zinc transport is questionable.

    Conclusions:

    • Amino acids are likely the primary low molecular weight zinc binders in Viokase.
    • The role of citrate and picolinate as zinc transporters in Viokase is significantly reduced.
    • Enhanced zinc uptake by citrate or picolinate is unlikely to be responsible for patient symptom relief in variant AE.

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