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Related Experiment Videos

Platelet-derived growth factor-modulated translatable mRNAs.

S L Hendrickson, C D Scher

    Molecular and Cellular Biology
    |August 1, 1983
    PubMed
    Summary

    Platelet-derived growth factor (PDGF) rapidly increases specific messenger RNA (mRNA) in cells before DNA synthesis. This mRNA accumulation is crucial for growth factor-stimulated cell division.

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    Area of Science:

    • Cell Biology
    • Molecular Biology
    • Biochemistry

    Background:

    • Platelet-derived growth factor (PDGF) is a key regulator of cell growth and division.
    • Understanding the molecular mechanisms by which PDGF stimulates DNA synthesis is crucial for cell biology research.

    Purpose of the Study:

    • To investigate the early molecular events triggered by PDGF in density-arrested BALB/c 3T3 cells.
    • To identify specific mRNAs whose accumulation is regulated by PDGF and epidermal growth factor (EGF).

    Main Methods:

    • Treatment of BALB/c 3T3 cells with purified PDGF and EGF.
    • Analysis of mRNA species using cell-free translation and gel electrophoresis.
    • Investigating the role of protein synthesis inhibition (cycloheximide) and RNA synthesis inhibition (actinomycin D).

    Main Results:

    • PDGF rapidly induced the accumulation of specific translatable mRNAs prior to DNA synthesis.
    • EGF also stimulated the accumulation of these mRNAs, but insulin and platelet-poor plasma did not.
    • Three classes of PDGF-modulated mRNAs were identified, differing in their timing and dependence on protein synthesis.
    • A transformed cell line constitutively accumulated these mRNAs, independent of exogenous growth factors.

    Conclusions:

    • The selective accumulation of specific mRNAs is an early, critical event in PDGF-stimulated DNA synthesis.
    • Growth factor-regulated mRNA accumulation may be a prerequisite for cell cycle progression.

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