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Inhibitory effect of Fusarium T2-toxin on lymphoid DNA and protein synthesis
Abstract:
The effect of T2-toxin on DNA and protein synthesis was investigated, both in vivo and in vitro. For mice which had been submitted to different treatment schedules (single dose, 3-, or 7-day treatment), the rate of DNA and protein biosynthesis was measured in thymus, bone marrow, liver, and spleen cells. T2-toxin inhibited DNA and protein synthesis, irrespectively of treatment schedule, in bone marrow, spleen, and thymus. In liver, after 3-day treatment, DNA and protein synthesis were both enhanced as compared to control values, whereas after 7-day treatment, only protein synthesis values remained greater than those of controls, and DNA synthesis was inhibited. After 7-day treatment, ornithine decarboxylase activity was inhibited in the thymus. In vitro, T2-toxin inhibited DNA and protein synthesis of mice spleen cells stimulated with phytohemagglutinin and rat hepatoma cells.
Insights
T2-toxin inhibits DNA and protein synthesis in mouse thymus, spleen, and bone marrow. Liver synthesis is initially enhanced but later inhibited by T2-toxin, impacting cellular functions.
Area of Science:
- Toxicology
- Molecular Biology
- Biochemistry
Background:
- T2-toxin is a mycotoxin with known cytotoxic effects.
- Understanding its impact on cellular synthesis is crucial for risk assessment.
Purpose of the Study:
- To investigate the in vivo and in vitro effects of T2-toxin on DNA and protein synthesis.
- To determine the influence of different T2-toxin treatment schedules on biosynthesis rates.
Main Methods:
- Mice were subjected to single, 3-day, or 7-day T2-toxin treatment schedules.
- DNA and protein biosynthesis rates were measured in thymus, bone marrow, liver, and spleen cells.
- In vitro assays examined T2-toxin's effect on stimulated mouse spleen cells and rat hepatoma cells.
Main Results:
- T2-toxin inhibited DNA and protein synthesis in bone marrow, spleen, and thymus across all schedules.
- Liver exhibited enhanced DNA and protein synthesis after 3-day treatment, with only protein synthesis remaining elevated after 7 days.
- T2-toxin inhibited DNA synthesis in the liver and ornithine decarboxylase activity in the thymus after 7-day treatment.
- In vitro, T2-toxin inhibited DNA and protein synthesis in stimulated mouse spleen and rat hepatoma cells.
Conclusions:
- T2-toxin exhibits dose- and tissue-dependent effects on DNA and protein synthesis.
- The toxin generally suppresses biosynthesis in immune and hematopoietic tissues.
- T2-toxin's impact on liver synthesis is complex, showing initial enhancement followed by inhibition.