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An inexpensive, microcomputer-based, video densitometer for quantitating thin-layer chromatographic spots.

T S Ford-Holevinski, B W Agranoff, N S Radin

    Analytical Biochemistry
    |July 1, 1983
    PubMed
    Summary
    This summary is machine-generated.

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    A new, affordable video densitometer was created using an Apple II microcomputer. This device accurately quantifies lipid and colored spots on chromatography plates, even with minor imperfections.

    Area of Science:

    • Analytical Chemistry
    • Biochemistry

    Background:

    • Thin-layer chromatography (TLC) is a common separation technique.
    • Accurate quantification of separated components is crucial for analysis.
    • Existing densitometry methods can be expensive or lack precision.

    Purpose of the Study:

    • To develop an inexpensive and accessible video densitometer for quantitative analysis of TLC plates.
    • To provide a reliable method for quantifying lipid and colored spots on various TLC plates.

    Main Methods:

    • Development of a video densitometer using an Apple II microcomputer and readily available components.
    • Utilizing the system to analyze charred lipid and colored spots on TLC plates.
    • Implementing software for automatic baseline correction to account for plate irregularities.

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    Main Results:

    • The developed densitometer accurately quantifies lipid samples in the microgram range (0.1-4 µg on high-performance plates, 1-20 µg on ordinary plates).
    • The system demonstrates robustness by automatically correcting for baseline drift caused by plate imperfections.
    • The method offers a cost-effective alternative for TLC plate quantification.

    Conclusions:

    • An affordable and effective video densitometer for TLC plate analysis has been successfully developed.
    • This system provides accurate quantification of lipid and colored spots, enhancing analytical capabilities.
    • The automated baseline correction feature improves reliability and ease of use for researchers.