High yield purification of protein 4.1 from human erythrocyte membranes

Protein 4.1 has been purified from human erythrocyte membranes by a simple method employing the nonionic detergent Tween 20 and anion exchange chromatography. The procedure results in the production of large quantities of protein 4.1, which retains immunoreactivity and the functional properties of binding to spectrin and enhancing the interaction of spectrin and actin.