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[Fluorometric method for the assay of IgG produced in lymphocyte cultures]
Bollettino Della Societa Italiana Di Biologia Sperimentale
|September 30, 1983
Abstract:
We have studied the IgG secreted in a system of CML. Microquantity of IgG (30-3000 micrograms/ml) of culture was determined using a fluorometric method based on the determination of the fluorescence derived by immune-complex IgG-FITC labeled goat Anti-human antibody to IgG covalently coupled to hydrophilic beads. This techniques has showed a good sensibility and accuracy.