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Related Experiment Videos

Epithelial membrane antigen: partial purification, assay and properties.

M G Ormerod, K Steele, J H Westwood

    British Journal of Cancer
    |October 1, 1983
    PubMed
    Summary

    Epithelial Membrane Antigen (EMA), a marker for detecting cancer cells, was purified from human milk. This soluble EMA is a heterogeneous glycoprotein, primarily composed of carbohydrates.

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    Area of Science:

    • Immunology
    • Biochemistry
    • Tumor Pathology

    Background:

    • Epithelial Membrane Antigen (EMA) is an immunological marker found on human non-squamous epithelial surfaces.
    • EMA is crucial in diagnostic tumor pathology for detecting malignant cell deposits in organs like the liver and bone marrow.

    Purpose of the Study:

    • To describe the purification and characterization of soluble Epithelial Membrane Antigen (EMA) from human milk.
    • To investigate the biochemical properties of purified EMA.

    Main Methods:

    • Purification of EMA from human milk.
    • Analysis of molecular weight, carbohydrate and protein composition, and inorganic content of EMA.

    Main Results:

    • Soluble EMA was successfully purified from human milk, revealing heterogeneous molecular weights.
    • The purified EMA is predominantly a carbohydrate structure, with galactose and N-acetylglucosamine as major sugars.
    • EMA contains low protein content with variable amino acid composition and a high inorganic material content.

    Conclusions:

    • Human milk is a viable source for EMA purification.
    • EMA is a complex glycoprotein with significant carbohydrate components.
    • Further characterization of EMA's composition is necessary.

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