Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Diffusion in lipid bilayers containing barriers.

A A Rigos, D F Calef, J M Deutch

    Biophysical Journal
    |September 1, 1983
    PubMed
    Summary
    This summary is machine-generated.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    Joint density-functional theory: ab initio study of Cr2O3 surface chemistry in solution.

    The journal of physical chemistry. B·2006
    Same author

    Conformational interconversion in protein crystals.

    Journal of molecular biology·1992
    Same author

    Response: ICBM Modernization.

    Science (New York, N.Y.)·1989
    Same author

    The role of polymer matrix structure and interparticle interactions in diffusion-limited drug release.

    Biophysical journal·1985
    Same author

    Structure-activity relationships in papain and bromelain ligand interactions.

    Archives of biochemistry and biophysics·1977
    Same author

    Frictional properties of nonspherical multisubunit structures. Application to tubules and cylinders.

    Biopolymers·1976
    Same journal

    Quantifying the Peripheral Surface Information Entropy from Conformational Ensembles of Globular Protein-Peptide Complexes.

    Biophysical journal·2026
    Same journal

    Anisotropic unbinding and location-dependent hovering of a kinesin motor head over microtubule.

    Biophysical journal·2026
    Same journal

    Kinesin-5/Cut7 C-terminal tail phosphorylation influence on motor regulation through multi-scale molecular modeling.

    Biophysical journal·2026
    Same journal

    Dynamic conformations of fluorophores on self-labeling protein tags.

    Biophysical journal·2026
    Same journal

    Different actions of RyR2 open and closed channel block explained by a multiscale Ca<sup>2+</sup> release model.

    Biophysical journal·2026
    Same journal

    Membrane Environment Sets the Functional pK<sub>a</sub> of Ionizable Lipids.

    Biophysical journal·2026
    See all related articles

    Tight junctions in epithelial cells restrict lipid probe diffusion. Theoretical models suggest experimental distinction between barrier crossing and flip-flop mechanisms is challenging, requiring significant flip-flop rate differences.

    Area of Science:

    • Cell Biology
    • Biophysics
    • Membrane Transport

    Background:

    • Epithelial cells possess tight junctions that act as barriers, limiting lipid probe diffusion across the outer membrane bilayer.
    • Understanding lipid probe diffusion is crucial for studying membrane dynamics and cell polarity.

    Purpose of the Study:

    • To theoretically investigate lipid probe diffusion across epithelial cell tight junctions.
    • To compare two models of tight junction function: a penetrable barrier and an impenetrable obstacle requiring flip-flopping.

    Main Methods:

    • Utilized the diffusion equation on planar and spherical surfaces to model tight junction barriers.
    • Calculated the rate of probe passage between cell sides under steady-state conditions for both models.
    • Compared theoretical predictions with experimental data from fluorescent photobleaching recovery experiments.

    Related Experiment Videos

    Main Results:

    • Two models for tight junction function were analyzed: a penetrable barrier and an impenetrable obstacle necessitating flip-flopping.
    • Theoretical calculations determined the rate of probe passage for each model.
    • Results indicated experimental difficulty in distinguishing between barrier crossing and flip-flop mechanisms.

    Conclusions:

    • Distinguishing between tight junction barrier crossing and lipid flip-flop mechanisms experimentally is challenging.
    • Explaining experimental results within a flip-flop model necessitates significant variations in probe flip-flop rates.
    • The study provides theoretical insights into lipid probe diffusion dynamics at epithelial tight junctions.