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Ca2+-sensitive permeability changes caused by influenza virus.

K Patel, C A Pasternak

    Bioscience Reports
    |August 1, 1983
    PubMed
    Summary
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    Influenza virus at low pH causes cell membrane changes, allowing ion and phosphorylated compound leakage, similar to Sendai virus effects. Cells remain intact and impermeable to trypan blue.

    Area of Science:

    • Virology
    • Cell Biology
    • Membrane Biophysics

    Background:

    • Viruses can alter host cell membrane permeability.
    • Sendai virus is known to induce membrane changes at neutral pH.
    • Understanding viral-induced membrane permeability is crucial for virology research.

    Purpose of the Study:

    • To investigate the effect of influenza virus on Lettré cell membrane permeability.
    • To compare the permeability changes induced by influenza virus at low pH with those induced by Sendai virus at neutral pH.

    Main Methods:

    • Treatment of Lettré cells with influenza virus at pH 5.3.
    • Treatment of Lettré cells with Sendai virus at pH 7.4 (implied comparison).
    • Monitoring of ion (K+, Na+) equilibration across the plasma membrane.

    Related Experiment Videos

  • Assessing the leakage of low-molecular-weight phosphorylated compounds.
  • Testing cell impermeability to trypan blue.

    • Main Results:

    • Influenza virus at pH 5.3 induced K+ and Na+ equilibration across the plasma membrane.
    • Low-molecular-weight phosphorylated compounds leaked out of influenza virus-treated cells.
    • The observed changes were similar to those elicited by Sendai virus at pH 7.4.
    • Cells treated with influenza virus remained impermeable to trypan blue.

    Conclusions:

    • Influenza virus, under acidic conditions (pH 5.3), can induce significant plasma membrane permeability changes in Lettré cells.
    • These changes involve ion flux and leakage of intracellular phosphorylated compounds.
    • The mechanism of membrane perturbation by influenza virus at low pH shares similarities with Sendai virus-induced effects at neutral pH.
    • The integrity of the cell membrane, as indicated by trypan blue exclusion, is maintained despite these permeability alterations.