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Trace elements in human seminal plasma and spermatozoa.

P A Pleban, D S Mei

    Clinica Chimica Acta; International Journal of Clinical Chemistry
    |September 15, 1983
    PubMed
    Summary

    New methods accurately measure trace elements like cadmium and lead in human semen. These findings are crucial for understanding male infertility and reproductive health.

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    Area of Science:

    • Reproductive Biology
    • Analytical Chemistry
    • Environmental Health

    Background:

    • Trace elements play a vital role in male reproductive function.
    • Accurate quantification of seminal trace elements is essential for infertility diagnostics.
    • Previous methodologies may lack precision or require extensive sample preparation.

    Purpose of the Study:

    • To develop and validate robust methodologies for analyzing essential and toxic trace elements in human seminal plasma and spermatozoa.
    • To assess the stability of trace elements in seminal plasma over time.
    • To determine trace element profiles in men undergoing infertility evaluations.

    Main Methods:

    • Zeeman-effect atomic absorption spectroscopy was employed for direct analysis of seminal plasma or digested spermatozoa.
    • Methodologies were validated using pooled specimens to determine within-run and between-run coefficients of variation.
    • Trace element concentrations were measured at various time points post-emission to assess stability.

    Main Results:

    • Developed reliable methods with within-run coefficients of variation from 0.5% to 9% and between-run coefficients of variation from 4% to 13%.
    • Demonstrated no significant change in seminal plasma trace element concentrations upon standing with spermatozoa for up to 5 hours.
    • Quantified trace element levels in patient specimens relevant to infertility studies.

    Conclusions:

    • Validated Zeeman-effect atomic absorption spectroscopy provides a reliable method for human seminal trace element analysis.
    • Seminal plasma trace element concentrations are stable post-emission, simplifying sample handling.
    • These analytical advancements support further research into the role of trace elements in male infertility.

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