Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Electrophysiologic differences between mouse extensor digitorum longus and soleus.

J A Florendo, J F Reger, P K Law

    Experimental Neurology
    |November 1, 1983
    PubMed
    Summary
    This summary is machine-generated.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    Cuticular composition and DDT resistance in the tobacco budworm.

    Journal of economic entomology·2010
    Same author

    Skeletal myoblast transplantation for attenuation of hyperglycaemia, hyperinsulinaemia and glucose intolerance in a mouse model of type 2 diabetes mellitus.

    Diabetologia·2009
    Same author

    Use of developmental language scales in Chinese children.

    Brain & development·2004
    Same author

    Effectiveness of transient immunosuppression using cyclosporine for xenomyoblast transplantation for cardiac repair.

    Transplantation proceedings·2004
    Same author

    Angiomyogenesis using human myoblast carrying human VEGF165 for injured heart.

    Annals of the Academy of Medicine, Singapore·2004
    Same author

    Human gene therapy with myoblast transfer.

    Transplantation proceedings·1997

    Fast- and slow-twitch muscle fibers can be distinguished by their electrophysiologic properties. Miniature end-plate potentials (MEPPs) and action potentials differ significantly between extensor digitorum longus (EDL) and soleus (SOL) muscle fibers.

    Area of Science:

    • Neuroscience
    • Muscle Physiology

    Background:

    • Muscle fibers are broadly classified as fast-twitch or slow-twitch.
    • Distinguishing between these fiber types is crucial for understanding muscle function and disease.

    Purpose of the Study:

    • To electrophysiologically differentiate between fast-twitch (extensor digitorum longus - EDL) and slow-twitch (soleus - SOL) muscle fibers in vivo.
    • To identify specific electrophysiological parameters that reliably distinguish EDL from SOL fibers.

    Main Methods:

    • In vivo electrophysiological recordings were performed on surface fibers of EDL and SOL muscles from Bar Harbor 129 mice.
    • Measurements included miniature end-plate potentials (MEPPs) and indirectly elicited action potentials at 37°C.
    • Key parameters analyzed were MEPP frequency, amplitude, duration, and action potential amplitude and duration.

    Related Experiment Videos

    Main Results:

    • EDL muscle fibers exhibited significantly higher MEPP frequency, smaller MEPP amplitude, and shorter MEPP duration compared to SOL fibers.
    • EDL action potentials showed significantly greater amplitude and shorter duration than SOL action potentials.
    • A single stimulus evoked multiple action potentials in EDL fibers, but only one in SOL fibers.
    • No significant differences were found in resting membrane potentials between EDL and SOL fibers.

    Conclusions:

    • Electrophysiological parameters of MEPPs and action potentials serve as reliable markers for distinguishing fast-twitch (EDL) and slow-twitch (SOL) muscle fibers.
    • These findings provide a basis for identifying and differentiating muscle fiber types based on their electrical activity.
    • The study highlights distinct electrophysiological profiles that correlate with the functional characteristics of fast- and slow-twitch muscles.