[Maturation of neuronal elements of the cerebellum in tissue culture]

The morphological and electrophysiological indices of the process of nervous cells differentiation were observed in the organotypical cerebellar culture of the newborn mice. The cerebellar cells reached the morphological maturity in the 18-21 day culture. The membrane potential augmentation began in the 6-7 day culture and reached the magnitude typical for the definitive stage of neuron (65-75 mV), in the 9-10 day culture. Spontaneous APs were recorded only in the 10-12 day culture, their characteristics being sharply different from those of the mature neuron. The difference reduced by the 16-18th day. The final maturity of cerebellar neurons, however as indicated by the AP, occurred still later and correlated with formation of the specific sensitivity of these cells to acetylcholine.