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Related Experiment Videos

Interactions between soluble IgG, complement and cells in lymphocyte and monocyte ADCC.

R N Poston, R S Morgan

    Immunology
    |November 1, 1983
    PubMed
    Summary

    Antibody-dependent cell-mediated cytotoxicity (ADCC) by K lymphocytes is inhibited by aggregated IgG, unlike monomeric IgG. Monocyte ADCC is inhibited by both forms, suggesting differences in Fc receptor affinity.

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    Area of Science:

    • Immunology
    • Cellular Biology
    • Biochemistry

    Background:

    • Antibody-dependent cell-mediated cytotoxicity (ADCC) is a crucial immune mechanism involving cytotoxic cells recognizing antibody-coated targets.
    • Two primary effector cells in ADCC are K lymphocytes and monocytes, differing in their Fc receptor characteristics and interactions with IgG.
    • Understanding these interactions is vital for comprehending immune responses and developing targeted therapies.

    Purpose of the Study:

    • To compare the functional interactions of K lymphocyte- and monocyte-mediated ADCC with monomeric and aggregated IgG.
    • To investigate the influence of IgG form and complement on ADCC activity.
    • To elucidate the role of Fc receptor affinity in differential ADCC responses.

    Main Methods:

    • Comparative analysis of K lymphocyte and monocyte ADCC assays.

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  • Incubation with monomeric and aggregated human IgG.
  • Assessment of inhibition by IgG, complement-bound aggregates, and adherent cells.

    • Main Results:

    • K lymphocyte ADCC was inhibited by aggregated IgG but not monomeric IgG.
    • Monocyte ADCC was inhibited by both monomeric and aggregated IgG, suggesting high-affinity Fc receptors.
    • Complement binding reduced aggregate-induced inhibition of K lymphocyte ADCC; adherent cells inhibited K lymphocyte ADCC only without IgG.

    Conclusions:

    • K lymphocyte ADCC exhibits properties consistent with a significant in vivo role due to differential sensitivity to IgG forms.
    • Monocyte ADCC is strongly inhibited by physiological IgG concentrations, potentially limiting its in vivo efficacy.
    • Differences in Fc receptor affinity likely underlie the distinct ADCC profiles of K lymphocytes and monocytes.