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Polyions regulate the alternative amplification pathway of complement.

J M Weiler

    Immunopharmacology
    |October 1, 1983
    PubMed
    Summary
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    Polycations and polyanions directly regulate complement alternative pathway convertase. Polycations inhibit convertase generation and modulate polyanion effects on complement activation.

    Area of Science:

    • Immunology
    • Biochemistry

    Background:

    • Polyanions inhibit complement amplification pathway convertase generation and decay.
    • The role of polycations in complement regulation is less understood.

    Purpose of the Study:

    • To investigate the direct effects of polycations (protamine sulfate, poly-l-lysine) on alternative amplification pathway mechanisms.
    • To determine how polycations regulate polyanion-induced inhibition of complement.

    Main Methods:

    • Assessing polycation effects on the generation of complement convertases (EAC4b,3b,Bb and EAC4b,3b,Bb,P).
    • Evaluating polycation interference with factor B consumption and binding to complement complexes.
    • Examining polycation influence on polyanion (heparin) mediated inhibition and decay of convertase.

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    Main Results:

    • Polycations inhibited the generation of EAC4b,3b,Bb and EAC4b,3b,Bb,P in a dose-dependent manner.
    • Polycations augmented the generation of EAC4b,3b,B at lower doses and inhibited at higher doses.
    • Polycations diminished polyanion inhibition of convertase generation but enhanced polyanion-induced decay of preformed convertase.

    Conclusions:

    • Both polycations and polyanions directly regulate complement alternative amplification pathway mechanisms.
    • Polycations exhibit complex regulatory effects on complement convertase formation and function.