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Reverse phase high pressure liquid chromatographic determination of vitamin K1 in infant formulas
Abstract:
A reverse phase high pressure liquid chromatographic (HPLC) method for quantitating vitamin K1 in enzymatic hydrolysates of infant formula is described. The vitamin is extracted with n-pentane before determination by isocratic and isothermal reverse phase HPLC. Recovery of vitamin K1 added to 5 infant formulas ranged from 84 to 103%.