Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Isolation of human serum spreading factor.

D W Barnes, J Silnutzer

    The Journal of Biological Chemistry
    |October 25, 1983
    PubMed
    Summary

    Researchers isolated human serum spreading factor (SF), a protein mixture, using advanced chromatography. This purified SF promotes cell spreading effectively at low concentrations.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    Characterization of human plasma growth inhibitory activity on serum-free mouse embryo cells.

    In vitro cellular & developmental biology. Animal·2016
    Same author

    Distribution of fission products in animals at Maralinga.

    Progress in nuclear energy. Series 6 Biological sciences·2014
    Same author

    Spontaneous and induced changes in cell populations in heavily irradiated mice.

    Progress in nuclear energy. Series 6 Biological sciences·2014
    Same author

    Cell and molecular biology of the spiny dogfish Squalus acanthias and little skate Leucoraja erinacea: insights from in vitro cultured cells.

    Journal of fish biology·2012
    Same author

    Culture of cells from two life stages of Schistosoma mansoni.

    Cytotechnology·2012
    Same author

    Transfection of cultured fish cells with exogenous DNA.

    Cytotechnology·2012

    Area of Science:

    • Biochemistry
    • Cell Biology

    Background:

    • Serum spreading factor (SF) plays a role in cell adhesion and spreading.
    • Understanding the purification and properties of SF is crucial for its applications.

    Purpose of the Study:

    • To isolate and purify human serum spreading factor (SF).
    • To characterize the molecular properties and activity of the purified SF.

    Main Methods:

    • Affinity chromatography using glass beads, concanavalin A-Sepharose, DEAE-agarose, and heparin-agarose.
    • Assays for cell spreading-promoting activity.
    • Molecular weight determination via SDS-PAGE.

    Main Results:

    • Human SF was purified approximately 260-fold.
    • The purified SF preparation consisted of two proteins, SF65 and SF75 (approx. 65,000 and 75,000 Da).
    • Maximal cell spreading activity was observed at 300 ng/ml; both SF65 and SF75 exhibited this activity.

    Conclusions:

    • A robust four-step purification protocol for human SF was established.
    • The purified SF is composed of two distinct protein components with cell spreading-promoting properties.
    • The study confirms the role of SF65 and SF75 in cell adhesion and spreading.

    Related Experiment Videos