Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Ascorbic acid and catecholamine secretion from cultured chromaffin cells.

M Levine, A Asher, H Pollard

    The Journal of Biological Chemistry
    |November 10, 1983
    PubMed
    Summary

    Ascorbic acid and catecholamines are secreted together from adrenal chromaffin cells. However, the study reveals ascorbic acid originates from both granules and cytoplasm, unlike catecholamines which are primarily granule-bound.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    Generalizability of a Musculoskeletal Therapist Electronic Health Record for Modelling Outcomes to Work-Related Musculoskeletal Disorders.

    Journal of occupational rehabilitation·2024
    Same author

    Identifying the suite of genes central to swimming in the biocontrol bacterium <i>Pseudomonas protegens</i> Pf-5.

    Microbial genomics·2024
    Same author

    Electronic Health Records for Predicting Outcomes to Work-Related Musculoskeletal Disorders: A Scoping Review.

    Journal of occupational rehabilitation·2024
    Same author

    Cyclin E and FGF8 are downstream cell growth regulators in distinct tumor suppressor effects of ANXA7 in hormone-resistant cancer cells of breast versus prostate origin.

    Trends in cancer research·2018
    Same author

    Consistency of feed efficiency ranking and mechanisms associated with inter-animal variation among growing calves.

    Journal of animal science·2018
    Same author

    "Chrono-functional milk": The difference between melatonin concentrations in night-milk versus day-milk under different night illumination conditions.

    Chronobiology international·2015

    Area of Science:

    • Neuroscience
    • Cell Biology
    • Biochemistry

    Background:

    • Bovine adrenal chromaffin cells are a model for studying stimulus-secretion coupling.
    • Chromaffin granules store and release catecholamines.
    • The intracellular source of secreted ascorbic acid in these cells is not fully understood.

    Purpose of the Study:

    • To investigate the cellular source of ascorbic acid secretion from bovine adrenal chromaffin cells.
    • To compare the secretion mechanisms of ascorbic acid and catecholamines.
    • To determine if ascorbic acid and catecholamines originate from the same intracellular compartments during secretion.

    Main Methods:

    • Cultured bovine adrenal chromaffin cells were preloaded with (R)-[14C]ascorbic acid.
    • Cells were stimulated with various secretagogues (veratridine, nicotine, acetylcholine, KCl).

    Related Experiment Videos

  • Secretion of labeled ascorbic acid and endogenous catecholamines was measured; subcellular fractions were analyzed.

    • Main Results:

    • Ascorbic acid and catecholamines were secreted concomitantly in a calcium-dependent manner.
    • While 95% of catecholamines were in chromaffin granules, only 16% of labeled ascorbic acid was found there.
    • Secreted ascorbic acid exceeded the amount expected from granule stores alone, indicating a cytoplasmic source.

    Conclusions:

    • Ascorbic acid and catecholamines are co-secreted from adrenal chromaffin cells.
    • Ascorbic acid secretion involves both chromaffin granules and a cytoplasmic pool.
    • The distinct intracellular origins suggest partially independent secretion pathways for ascorbic acid and catecholamines.